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 | Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA La Estanzuela; INIA Las Brujas. |
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Fecha : |
18/09/2014 |
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Actualizado : |
25/11/2019 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
CASTILLO, A.; REBUFFO, M.; DALLA RIZZA, M.; FOLLE, G.; SANTIÑAQUE, F.; BORSANI, O.; MONZA, J. |
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Afiliación : |
ALICIA MARIA CASTILLO SALLE, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay; MONICA IRENE REBUFFO GFELLER, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay; MARCO DALLA RIZZA VILARO, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay. |
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Título : |
Generation and characterization of interspecific hybrids of Lotus uliginosus × Lotus corniculatus. |
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Fecha de publicación : |
2012 |
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Fuente / Imprenta : |
Crop Science, 2012, v. 52, no. 4, p. 1572-1582. |
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ISSN : |
0011-183X |
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DOI : |
10.2135/cropsci2011.07.0374 |
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Idioma : |
Inglés |
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Notas : |
Article history: Published July, 2012. |
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Contenido : |
ABSTRACT.
A method is described for obtaining interspecific hybrids between commercial lines of Lotus uliginosus Schkuhr and Lotus corniculatus L. Hybridization was possible between these species using embryo rescue. Two strategies were used to confirm 40 F1 hybrids, that is, flow cytometry and microsatellite simple sequence repeats (SSRs). The L. uliginosus and L. corniculatus parents have significantly different genome size (2.5 ± 0.02 and 2.22 ± 0.02 pg, respectively) and the offspring from the cross had intermediate values. Two SSR primers were polymorphic between the genotypes tested, TM1150 and EH380069. Maternal effect was detected in L. uliginosus genotypes, as determined by greater frequency of F1 hybrids. Recombinant traits were observed in the F1 hybrid progeny. Ninety F1 hybrids resulting from L. uliginosus × L. corniculatus crosses showed different levels of fertility in the polycross; <3% F1 plants produced more than 1000 seeds without embryo rescue while 10% of plants did not flower the first year. The F1 hybrid plants grown under greenhouse conditions exhibited shoot phenotypes similar to L. uliginosus (maternal) whereas F2 phenotypes were similar to L. corniculatus (paternal) in field trials. Rhizome presence was observed in 60% of F2 hybrid plants. The diameters of the root crowns of these plants, however, were similar to that of L. corniculatus.
© Crop Science Society of America. |
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Palabras claves : |
LEGUMINOSAS FORRAJERAS; MARCADORES MOLECULARES. |
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Thesagro : |
CULTIVOS; PLANTAS FORRAJERAS. |
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Asunto categoría : |
--
F01 Cultivo |
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Marc : |
LEADER 02262naa a2200277 a 4500
001 1050609
005 2019-11-25
008 2012 bl uuuu u00u1 u #d
022 $a0011-183X
024 7 $a10.2135/cropsci2011.07.0374$2DOI
100 1 $aCASTILLO, A.
245 $aGeneration and characterization of interspecific hybrids of Lotus uliginosus × Lotus corniculatus.$h[electronic resource]
260 $c2012
500 $aArticle history: Published July, 2012.
520 $aABSTRACT. A method is described for obtaining interspecific hybrids between commercial lines of Lotus uliginosus Schkuhr and Lotus corniculatus L. Hybridization was possible between these species using embryo rescue. Two strategies were used to confirm 40 F1 hybrids, that is, flow cytometry and microsatellite simple sequence repeats (SSRs). The L. uliginosus and L. corniculatus parents have significantly different genome size (2.5 ± 0.02 and 2.22 ± 0.02 pg, respectively) and the offspring from the cross had intermediate values. Two SSR primers were polymorphic between the genotypes tested, TM1150 and EH380069. Maternal effect was detected in L. uliginosus genotypes, as determined by greater frequency of F1 hybrids. Recombinant traits were observed in the F1 hybrid progeny. Ninety F1 hybrids resulting from L. uliginosus × L. corniculatus crosses showed different levels of fertility in the polycross; <3% F1 plants produced more than 1000 seeds without embryo rescue while 10% of plants did not flower the first year. The F1 hybrid plants grown under greenhouse conditions exhibited shoot phenotypes similar to L. uliginosus (maternal) whereas F2 phenotypes were similar to L. corniculatus (paternal) in field trials. Rhizome presence was observed in 60% of F2 hybrid plants. The diameters of the root crowns of these plants, however, were similar to that of L. corniculatus. © Crop Science Society of America.
650 $aCULTIVOS
650 $aPLANTAS FORRAJERAS
653 $aLEGUMINOSAS FORRAJERAS
653 $aMARCADORES MOLECULARES
700 1 $aREBUFFO, M.
700 1 $aDALLA RIZZA, M.
700 1 $aFOLLE, G.
700 1 $aSANTIÑAQUE, F.
700 1 $aBORSANI, O.
700 1 $aMONZA, J.
773 $tCrop Science, 2012$gv. 52, no. 4, p. 1572-1582.
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